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KMID : 0364820130490030297
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Korean Journal of Microbiology
2013 Volume.49 No. 3 p.297 ~ p.300
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Isolation and Characteristics of Exopolysaccharide Producing Bacteria in a Ginseng Root System
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Cho Geon-Yeong
Jeon In-Hwa
Han Song-Ih
Whang Kyung-Sook
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Abstract
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EPS producing bacteria were enumerated in ginseng root system (rhizosphere soil, rhizoplane, inside of root). EPS
producing bacterial density of rhizosphere soil, rhizoplane and inside of root were distributed 9.0×106CFU/g,7.0×106
CFU/g, and 1.4×103CFU/g, respectively. Phylogenetic analysis of the 24 EPS producing isolates based on
the 16S rRNA gene sequences, EPS producing isolates from rhizosphere soil (RS) belong to genus Arthrobacter (6
strains) and Rhizobium (1 strain). EPS producing bacteria from rhizoplane (RP) were Arthrobacter(6 strains),Rhodococcus (1 strain) and Pseudomonas (1 strain). EPS producing bacteria from inside of root (IR) were categorized into Rhzobium (6 strains), Bacillus (1 strain), Rhodococcus (1 strain), and Pseudomonas (1 strain).
Phylogenetic analysis indicated that Arthrobacter may be a member of representative EPS producing bacteria from
ginseng rhizosphere soil and rhizoplane, and Rhizobium is typical EPS producing isolates from inside of ginseng
root. The yield of EPS was 10.0 and 4.9 g/L by Rhizobium sp. 1NP2 (KACC 17637) and Arthrobacter sp. 5MP1
(KACC 17636). The purified EPS were analyzed by Bio-LC and glucose, galactose, mannose and glucosamine were
detected. The major EPS sugar of these strains was glucose (72.7-84.9%).
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KEYWORD
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Arthrobacter sp., Rhizobium sp., exopolysaccharide, ginseng root system
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